Bethyl Laboratories
Rabbit anti-SAPS2 Antibody Affinity Purified
Primary
Catalog #A300-970A
TargetSAPS2
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
Image 1 of 1
local_shipping
Order within 18 hours and 31 minutes for shipment by Tuesday, November 10
Product Description
SAPS2 is a regulatory subunit of the PP6 phosphatase. There are 3 human SAPs (sit4-associated proteins), SAPS1, SAPS2, and SAPS3. The human SAPs are related to SAPs found in yeast that associate with the sit4 phosphatase which is the homolog of human PP6 phosphatase. In yeast sit4 and its associated SAPs are involved in a variety of processes which include transcription, translation, bud formation, glycogen metabolism, ion homeostasis, and telomere function.SAPS2 has been identified as gene induced by beta1-integrin ligation on B-cells and by interleukin 13 in airway smooth muscle.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 916 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetSAPS2
Antigen Species
Human
Gene ID9701
Gene Symbol
PPP6R2
Gene Name
protein phosphatase 6 regulatory subunit 2
Uniprot IDO75170
Protein Name
Serine/threonine-protein phosphatase 6 regulatory subunit 2
Product Information
Applications
IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to SAPS2 immobilized on solid support.
The epitope recognized by A300-970A maps to a region between residue 875 and the C-terminus (residue 932) of human SAPS domain family, member 2 using the numbering given in entry NP_055493.2 (GeneID 9701).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A300-970A · Lot 1 (current lot)Open
Product DatasheetPDF · A300-970A · Lot 1 (current lot)Open
Safety Data SheetPDF · A300-970AOpen
Citations / Publications
Services
Antibody Conjugation
Antibody and conjugate formats developed together, built to your specification.
Request CustomizationCustom Monoclonal Antibody Discovery Services
Full custom monoclonal development, from immunization through validation.
Explore Monoclonal DevelopmentVHH Discovery
Single-domain antibodies for structurally difficult targets a conventional monoclonal can't reach.
Explore VHH DiscoveryResources
1