/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-SARA Antibody
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Primary Antibodies  

Rabbit anti-SARA Antibody

Bethyl Laboratories®
Bethyl Laboratories®

Catalog #

Validated
Target:

SARA

Reactivity:

Human

Applications:

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 1 and 50

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Additional Product Information

Smad anchor for receptor activation (SARA) is an early endosomal protein that functions to recruit SMAD2/SMAD3 to intracellular membranes and to the TGF-beta receptor. SARA plays a significant role in TGF-mediated signaling by regulating the subcellular location of SMAD2 and SMAD3 and modulating the transcriptional activity of the SMAD3/SMAD4 complex. SARA is possibly associated with TGF-beta receptor internalization [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/O95405].

9372

ZFYVE9

zinc finger FYVE-type containing 9

O95405

Zinc finger FYVE domain-containing protein 9

Alternate Names

hSARA; MAD, mothers against decapentaplegic homolog interacting protein, receptor activation anchor; MADH-interacting protein; MADHIP; Mothers against decapentaplegic homolog-interacting protein; novel serine protease; NSP; PPP1R173; protein phosphatase 1, regulatory subunit 173; Receptor activation anchor; SARA; smad anchor for receptor activation; SMADIP; zinc finger FYVE domain-containing protein 9; zinc finger, FYVE domain containing 9

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.