/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-SART1 Antibody
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Primary Antibodies  

Rabbit anti-SART1 Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Citations (1)
Target:

SART1

Reactivity:

Human

Applications:

IP

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 700 and 750

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

$121.00 $433.00
Qty:
Bulk Request

Product Details

Specifications
Verified Reactivity

Human

Presumed Reactivity

Mouse,

Rat

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Production Procedures

Antibody was affinity purified using an epitope specific to SART1 immobilized on solid support.

The epitope recognized by A301-422A-T maps to a region between residue 675 and 725 of human squamous cell carcinoma antigen recognized by T cells using the numbering given in entry NP_005137.1 (GeneID 9092).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Antibody was affinity purified using an epitope specific to SART1 immobilized on solid support.

The epitope recognized by A301-422A maps to a region between residue 675 and 725 of human squamous cell carcinoma antigen recognized by T cells using the numbering given in entry NP_005137.1 (GeneID 9092).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Country of Origin

USA

Additional Product Information

SART1 (squamous cell carcinoma antigen recognized by T cells 1) was originally identified as a gene that encodes an antigenic peptide expressed in squamous cell carcinomas and recognized by cytotoxic T lymphocytes. The SART1 gene encodes two proteins, SART1(800) and SART1(259). SART1(800) has been found to be expressed in the nucleus of the majority of proliferating cells and has been identified as a component of the splicesomal complex required for U4/U6.U5 tri-snRNP recruitment and the assembly of mature splicesomes.

9092

SART1

spliceosome associated factor 1, recruiter of U4/U6.U5 tri-snRNP

O43290

U4/U6.U5 tri-snRNP-associated protein 1

Alternate Names

allergen Hom s 1; Ara1; HAF; HOMS1; hSART-1; hSnu66; hypoxia associated factor; IgE autoantigen; SART-1; SART1(259) protein; SART1(800) protein; SART1, U4/U6.U5 tri-snRNP-associated protein 1; SART1259; small nuclear ribonucleoprotein 110kDa (U4/U6.U5); SNRNP110; Snu66; SNU66 homolog; squamous cell carcinoma antigen recognised by T cells; squamous cell carcinoma antigen recognized by T-cells 1; U4/U6.U5 tri-snRNP-associated 110 kDa protein; U4/U6.U5 tri-snRNP-associated protein 1

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.