Bethyl Laboratories
Rabbit anti-SART3/TIP110 Antibody Affinity Purified
Primary
Catalog #A301-522A
TargetSART3/TIP110
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
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Product Description
SART3 (squamous cell carcinoma antigen recognized by T cells 3) was identified as an antigenic peptide expressed in esophageal cancer cells that is recognized by cytotoxic T lymphocytes. Originally, in a search for a U6 small nuclear RNA capping enzyme, SART3 was identified as a 110kDa nuclear RNA-binding protein. Later, by database searching, SART 3 was determined to also be the human ortholog of the yeast Prp24 snRNP recycling factor that functions in the reassembly of the U4/U6 snRNP during the recycling phase of the splicesome cycle. Recently, SART3-derived peptides have been investigated as candidates for peptide-based immunotherapy in prostate cancer patients due to their ability to induce cytotoxic T lymphocyte activity against prostate cancer cells.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 913 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetSART3/TIP110
Antigen Species
Human
Gene ID9733
Gene Symbol
SART3
Gene Name
squamous cell carcinoma antigen recognized by T-cells 3
Uniprot IDQ15020
Protein Name
Squamous cell carcinoma antigen recognized by T-cells 3
Alternate Names
DSAP1 • HIV-1 Tat-interacting protein of 110kDa • hSART-3 • P100 • p110 • p110 nuclear RNA-binding protein • p110(nrb) • RP11-13G14 • SART-3 • squamous cell carcinoma antigen recognized by T cells 3 • squamous cell carcinoma antigen recognized by T-cells 3 • tat-interacting protein of 110 kDa • TIP110
Product Information
Applications
IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to SART3/TIP110 immobilized on solid support.
The epitope recognized by A301-522A maps to a region between residue 913 and 963 of human squamous cell carcinoma antigen recognized by T-cells 3 (Tat-interacting protein of 110 kDa) using the numbering given in entry NP_055521.1 (GeneID 9733).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A301-522A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-522A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-522AOpen
Citations / Publications
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