Bethyl Laboratories
Rabbit anti-SASH1 IHC Antibody Affinity Purified
Primary
Catalog #IHC-00646
TargetSASH1
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, IHC-IF
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Product Description
SASH1 (SAM and SH3 domain containing 1) was identified in an in silico analysis of loss of heterozygosity (LOH) to identify genes downregulated in breast cancer. Since this discovery, it has also been found to be a candidate tumor suppressor in colon cancer. Structurally, SASH1 contains two SAM (sterile alpha motif) domains and one SH3 domain indicating a role in signal transduction and as a signaling adapter.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 750 and 800
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
250 µg/ml
Target Identity
TargetSASH1
Antigen Species
Human
Gene ID23328
Gene Symbol
SASH1
Gene Name
SAM and SH3 domain containing 1
Uniprot IDO94885
Protein Name
SAM and SH3 domain-containing protein 1
Product Information
Applications
IHC, IHC-IF
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to SASH1 immobilized on solid support.
The epitope recognized by IHC-00646 maps to a region between residue 750 and 800 of human SAM and SH3 domain containing 1 using the numbering given in entry NP_056093.3 (GeneID 23328).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
Epitope exposure is recommended.
Epitope exposure with citrate buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.
Immunohistochemistry (IHC)1:100 - 1:500
Immunohistochemistry-Immunofluoresence (IHC-IF)1:50 - 1:500
Documents
Safety Data SheetPDF · IHC-00646Open
Product DatasheetPDF · IHC-00646 · Lot 1 (current lot)Open
Product DatasheetPDF · IHC-00646 · Lot 1 (current lot)Open
Services
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