Bethyl Laboratories

Rabbit anti-SCFD1 Antibody Affinity Purified

Primary
Catalog #A302-138A
TargetSCFD1
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP
Detection of human SCFD1 by western blot of immunoprecipitates.
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Product Description

SCFD1 (sec1 family domain containing 1) belongs to the STXBP-unc/SEC1 family of proteins. SEC1 proteins are involved in synaptic transmission and general secretion. SCFD1 is proposed to play a role in vesicular transport between the endoplasmic reticulum and the Golgi.

Specifications

Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 550 and 600
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetSCFD1
Antigen Species
Human
Gene ID23256
Gene Symbol
SCFD1
Gene Name
sec1 family domain containing 1
Uniprot IDQ8WVM8
Protein Name
Sec1 family domain-containing protein 1
Alternate Names
Product Information
Applications
IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA

Production & Epitope

Antibody was affinity purified using an epitope specific to SCFD1 immobilized on solid support.

The epitope recognized by A302-138A maps to a region between residue 5500 and 600 of human sec1 family domain containing 1 using the numbering given in entry NP_057190.2 (GeneID 23256).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.

Immunoprecipitation (IP)6 µg/mg lysate

Documents

Product DatasheetPDF · A302-138A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-138A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-138AOpen
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