Bethyl Laboratories
Rabbit anti-SEC63 Antibody Affinity Purified
Primary
Catalog #A305-084A
TargetSEC63
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIP, WB
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Product Description
The Sec61 complex is the central component of the protein translocation apparatus of the endoplasmic reticulum (ER) membrane. Translocation protein SEC63 homolog, encoded by the gene SEC63, and the SEC62 protein are found to be associated with ribosome-free SEC61 complex. It is speculated that Sec61-Sec62-Sec63 may perform post-translational protein translocation into the ER. The Sec61-Sec62-Sec63 complex might also perform the backward transport of ER proteins that are subject to the ubiquitin-proteasome-dependent degradation pathway [taken from NCBI Entrez Gene (Gene ID: 11231)].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 710 and C-terminus
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetSEC63
Antigen Species
Human
Gene ID11231
Gene Symbol
SEC63
Gene Name
SEC63 homolog, protein translocation regulator
Uniprot IDQ9UGP8
Protein Name
Translocation protein SEC63 homolog
Alternate Names
Product Information
Applications
IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to SEC63 immobilized on solid support.
The epitope recognized by A305-084A maps to a region between residue 700 to 750 of human Translocation protein SEC63 homolog using the numbering given in entry Q9UGP8.2 (GeneID 11231).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:10,000 - 1:25,000
Documents
Product DatasheetPDF · A305-084A · Lot 1 (current lot)Open
Product DatasheetPDF · A305-084A · Lot 1 (current lot)Open
Safety Data SheetPDF · A305-084AOpen
Citations / Publications
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