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Rabbit anti-SEMA4D Antibody

Bethyl Laboratories®

Bethyl Laboratories® Catalog #

Validated
Target:
SEMA4D
Reactivity:
Human
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 812 and 862
IsoType:
IgG
Purity:
Antigen Affinity Purified
100 µl (1000 µg/ml)

100 µl (10 blots)

20 µl (2 blots)

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Product Details

Specifications
Verified Reactivity
Human
Presumed Reactivity
Mouse
Antigen Species
Human
Stock Concentration
100 µg/ml
1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
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Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
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Prod Procedures
Antibody was affinity purified using an epitope specific to SEMA4D immobilized on solid support.

The epitope recognized by A304-395A-T maps to a region between residue 812 to 862 of human Sema Somain, Immunoglobulin Domain (Ig), Transmembrane Tomain (TM) and Short Cytoplasmic Domain, (Semaphorin) 4D using the numbering given in entry NP_006369.3 (GeneID 10507).

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Gene & Protein Information

Sema4D (Semaphorin-4D) is a cell surface receptor for PLXN1B and PLXNB2 that plays an important role in cell-cell signaling. Sema4D promotes reorganization of the actin cytoskeleton and plays a role in axonal growth cone guidance in the developing central nervous system. Sema4D regulates dendrite and axon branching and morphogenesis. It promotes the migration of cerebellar granule cells and of endothelial cells. In the immune system Sema4D induces B-cells to aggregate and improves their viability (in vitro). Sema4D also promotes signaling via SRC and PTK2B/PYK2, which then mediates activation of phosphatidylinositol 3-kinase and of the AKT1 signaling cascade [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q92854].

10507

SEMA4D

semaphorin 4D

Q92854

Semaphorin-4D

Alternate Names

A8, BB18, C9orf164, CD antigen CD100, CD100, COLL4, coll-4, GR3, M-sema-G, sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D, sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, 4D, SEMAJ, semaphorin-4D

Applications

Western blot of immunoprecipitates performed using Normal Pig Serum (Cat. No. S100-020), Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) and 4-8% SDS-PAGE. Western blot of lysates performed using standard western blot reagents and 4-8% SDS-PAGE.