Bethyl Laboratories
Rabbit anti-Septin 7 Antibody Affinity Purified
Primary
Catalog #A304-213A
TargetSeptin 7
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC, IP, WB
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Product Description
Septin 7 is a filament-forming cytoskeletal GTPase. Septin 7 is required for normal organization of the actin cytoskeleton and progress through mitosis. Septin 7 is involved in cytokinesis and is required for normal association of CENPE with the kinetochore. It also plays a role in ciliogenesis and collective cell movement [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q16181].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 387 and C-terminus
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetSeptin 7
Antigen Species
Human
Gene ID989
Gene Symbol
SEPT7
Gene Name
septin 7
Uniprot IDQ16181
Protein Name
Septin-7
Alternate Names
Product Information
Applications
IHC, IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to Septin 7 immobilized on solid support.
The epitope recognized by A304-213A maps to a region between residue 387 to 437 of human Septin 7 using the numbering given in entry NP_001779.3 (GeneID 989).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:500 - 1:2000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A304-213A · Lot 1 (current lot)Open
Product DatasheetPDF · A304-213A · Lot 1 (current lot)Open
Safety Data SheetPDF · A304-213AOpen
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