Bethyl Laboratories
Rabbit anti-Septin 9 Antibody Affinity Purified
Primary
Catalog #A302-353A
TargetSeptin 9
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, IP, WB
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Product Description
Septin 9 is a member of the septin family involved in cytokinesis and cell cycle control. This gene is a candidate for the ovarian tumor suppressor gene. Mutations in this gene cause hereditary neuralgic amyotrophy, also known as neuritis with brachial predilection. A chromosomal translocation involving this gene on chromosome 17 and the MLL gene on chromosome 11 results in acute myelomonocytic leukemia [taken from NCBI GeneID:10801].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 100 and 150
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetSeptin 9
Antigen Species
Human
Gene ID10801
Gene Symbol
SEPT9
Gene Name
septin 9
Uniprot IDQ9UHD8
Protein Name
Septin-9
Alternate Names
Product Information
Applications
IHC, IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to Septin 9 immobilized on solid support.
The epitope recognized by A302-353A maps to a region between residue 100 and 150 of human septin 9 using the numbering given in entry NP_001106963.1 (GeneID 10801).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A302-353A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-353A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-353AOpen
Citations / Publications
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