Bethyl Laboratories
Rabbit anti-Sequestosome-1 Antibody Affinity Purified
Primary
Catalog #A302-855A
TargetSequestosome-1
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC, IP, WB
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Product Description
Sequestosome-1 is an adapter protein that binds ubiquitin and may regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. Sequestosome-1 may play a role in titin/TTN downstream signaling in muscle cells, and may regulate signaling cascades through ubiquitination. Sequestosome-1 may be involved in cell differentiation, apoptosis, immune response and regulation of K+ channels [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ Q13501].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 325 and 375
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetSequestosome-1
Antigen Species
Human
Gene ID8878
Gene Symbol
SQSTM1
Gene Name
sequestosome 1
Uniprot IDQ13501
Protein Name
Sequestosome-1
Alternate Names
A170 • DMRV • EBI3-associated protein of 60 kDa • EBI3-associated protein p60 • EBIAP • FTDALS3 • NADGP • OSIL • oxidative stress induced like • p60 • p62 • p62B • PDB3 • phosphotyrosine independent ligand for the Lck SH2 domain p62 • phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa • sequestosome-1 • ubiquitin-binding protein p62 • ZIP3
Product Information
Applications
IHC, IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to Sequestosome-1 immobilized on solid support.
The epitope recognized by A302-855A maps to a region between residue 325 and 375 of human Sequestosome-1 using the numbering given in entry NP_003891.1 (GeneID 8878).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:500 - 1:2000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A302-855A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-855A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-855AOpen
Citations / Publications
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