Bethyl Laboratories
Rabbit anti-SF3a120/SAP114 Antibody Affinity Purified
Primary
Catalog #A301-602A
TargetSF3a120/SAP114
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC, IP, WB
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Product Description
SF3a120/SAP114 is a 120 kDa subunit of the heterotrimeric splicing factor 3a (SF3A) protein complex. SF3A along with SF3B and a 12S RNA subunit are components of the 17S U2 small nuclear ribonucleoprotein complex (U2 snRNP) involved in pre-mRNA splicing. SF3a120/SAP114 bears two SURP motif repeats and one ubiquitin-like domain. SURP motifs are found among proteins involved in regulated mRNA splicing. The SURP motifs along with the ubiquitin-like domain of SF3a120/SAP114 appear to function in the heterotrimeric formation between the 120, 60 and 66 kDa subunits.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 375 and 425
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetSF3a120/SAP114
Antigen Species
Human
Gene ID10291
Gene Symbol
SF3A1
Gene Name
splicing factor 3a subunit 1
Uniprot IDQ15459
Protein Name
Splicing factor 3A subunit 1
Alternate Names
Product Information
Applications
IHC, IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to SF3a120/SAP114 immobilized on solid support.
The epitope recognized by A301-602A maps to a region between residue 350 and 400 of human splicing factor 3a, subunit 1, 120kDa using the numbering given in entry NP_005868.1 (GeneID 10291).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:200 - 1:1000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A301-602A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-602A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-602AOpen
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