Bethyl Laboratories
Rabbit anti-SF3A3 Antibody Affinity Purified
Primary
Catalog #A302-506A
TargetSF3A3
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIP, WB
Image 1 of 1
local_shipping
Order within 13 hours and 40 minutes for shipment by today
Product Description
SF3A3 is one of 3 subunits of the SF3a splicing factor. SF3a is composed of a 60 kDa subunit (SF3A3/SF3a60), 66 kDa subunit (SF3A2/SF3a66), and a120 kDa subunit (SF3A1/SF3a120). SF3a is necessary for the in vitro conversion of 15S U2 snRNP into an active 17S particle that performs pre-mRNA splicing [taken from NCBI Entrez Gene (GeneID: 10946)].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 75 and 125
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetSF3A3
Antigen Species
Human
Gene ID10946
Gene Symbol
SF3A3
Gene Name
splicing factor 3a subunit 3
Uniprot IDQ12874
Protein Name
Splicing factor 3A subunit 3
Product Information
Applications
IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to SF3A3 immobilized on solid support.
The epitope recognized by A302-506A maps to a region between residue 75 and 125 of human splicing factor 3a, subunit 3, 60kDa using the numbering given in entry NP_006793.1 (GeneID 10946).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A302-506A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-506A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-506AOpen
Citations / Publications
Services
Antibody Conjugation
Antibody and conjugate formats developed together, built to your specification.
Request CustomizationCustom Monoclonal Antibody Discovery Services
Full custom monoclonal development, from immunization through validation.
Explore Monoclonal DevelopmentVHH Discovery
Single-domain antibodies for structurally difficult targets a conventional monoclonal can't reach.
Explore VHH DiscoveryResources
1