Bethyl Laboratories
Rabbit anti-SF3b145/SAP145 Antibody Affinity Purified
Primary
Catalog #A301-605A
TargetSF3b145/SAP145
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, IP, WB
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Product Description
SF3b145/SAP145 is a 145 kDa subunit of the splicing factor 3B (SF3B) protein complex. The SF3B complex is composed of at least 8 subunits (SF3B1 through SF3B8). SF3A along with SF3B and a 12S RNA unit are components of the 17S U2 small nuclear ribonucleoprotein complex (U2 snRNP) involved in pre-mRNA splicing. In this complex SF3B2 interacts directly with SF3B4 and associates with the pre-mRNA upstream of the branch site at the anchoring site. SF3A and SF3B may play a role in anchoring the U2 snRNP to the pre-mRNA upstream of the intron’s branch site.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 800 and 850
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetSF3b145/SAP145
Antigen Species
Human
Gene ID10992
Gene Symbol
SF3B2
Gene Name
splicing factor 3b subunit 2
Uniprot IDQ13435
Protein Name
Splicing factor 3B subunit 2
Alternate Names
Cus1 • pre-mRNA splicing factor SF3b 145 kDa subunit • Pre-mRNA-splicing factor SF3b 145 kDa subunit • SAP 145 • SAP145 • SF3b1 • SF3B145 • SF3b150 • spliceosome associated protein 145 • Spliceosome-associated protein 145 • splicing factor 3B subunit 2 • splicing factor 3b, subunit 2, 145kD • splicing factor 3b, subunit 2, 145kDa
Product Information
Applications
IHC, IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to SF3b145/SAP145 immobilized on solid support.
The epitope recognized by A301-605A maps to a region between residue 775 and 825 of human splicing factor 3b, subunit 2, 145kDa (spliceosome associated protein 145) using the numbering given in entry AAA97461.1 (GeneID 10992).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A301-605A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-605A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-605AOpen
Citations / Publications
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