/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-SF3b145/SAP145 IHC Antibody
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Primary Antibodies  

Rabbit anti-SF3b145/SAP145 IHC Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Target:

SF3b145/SAP145

Reactivity:

Human

,

Mouse

Applications:

IHC

,

IHC-IF

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 800 and 850

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (50+ slides)

10 µl (5+ slides)

$121.00 $459.00
Qty:
Bulk Request

Product Details

Specifications
Verified Reactivity

Mouse,

Human

Presumed Reactivity

Horse,

Dog,

Pig,

Rabbit,

Bovine,

Monkey,

Rat

Antigen Species

Human

Concentration
250 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide


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Production Procedures

Antibody was affinity purified using an epitope specific to SF3b145/SAP145 immobilized on solid support.

The epitope recognized by IHC-00317 maps to a region between residue 775 and 825 of human splicing factor 3b, subunit 2, 145kDa (spliceosome associated protein 145) using the numbering given in entry AAA97461.1 (GeneID 10992).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Antibody was affinity purified using an epitope specific to SF3b145/SAP145 immobilized on solid support.

The epitope recognized by IHC-00317-T maps to a region between residue 775 and 825 of human splicing factor 3b, subunit 2, 145kDa (spliceosome associated protein 145) using the numbering given in entry AAA97461.1 (GeneID 10992).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Country of Origin

USA

Additional Product Information

SF3b145/SAP145 is a 145 kDa subunit of the splicing factor 3B (SF3B) protein complex. The SF3B complex is composed of at least 8 subunits (SF3B1 through SF3B8). SF3A along with SF3B and a 12S RNA unit are components of the 17S U2 small nuclear ribonucleoprotein complex (U2 snRNP) involved in pre-mRNA splicing. In this complex SF3B2 interacts directly with SF3B4 and associates with the pre-mRNA upstream of the branch site at the anchoring site. SF3A and SF3B may play a role in anchoring the U2 snRNP to the pre-mRNA upstream of the intron’s branch site.

10992

SF3B2

splicing factor 3b subunit 2

Q13435

Splicing factor 3B subunit 2

Alternate Names

Cus1; pre-mRNA splicing factor SF3b 145 kDa subunit; Pre-mRNA-splicing factor SF3b 145 kDa subunit; SAP 145; SAP145; SF3b1; SF3B145; SF3b150; spliceosome associated protein 145; Spliceosome-associated protein 145; splicing factor 3B subunit 2; splicing factor 3b, subunit 2, 145kD; splicing factor 3b, subunit 2, 145kDa

Applications

Epitope exposure is recommended.

Epitope exposure with citrate buffer will enhance staining.

Likely to work with frozen sections.

In some cases, the antibody may be diluted further than indicated.