Bethyl Laboratories
Rabbit anti-SF3B4 Antibody Affinity Purified
Primary
Catalog #A303-950A
TargetSF3B4
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC, IP, WB
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Product Description
Splicing factor 3b, subunit 4, 49kDa (SF3B4) is one of four subunits of the splicing factor 3B. The protein encoded by this gene cross-links to a region in the pre-mRNA immediately upstream of the branchpoint sequence in pre-mRNA in the prespliceosomal complex A. It also may be involved in the assembly of the B, C and E spliceosomal complexes. In addition to RNA-binding activity, this protein interacts directly and highly specifically with subunit 2 of the splicing factor 3B [taken from NCBI Entrez Gene (Gene ID: 10262)].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 374 and C-terminus
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetSF3B4
Antigen Species
Human
Gene ID10262
Gene Symbol
SF3B4
Gene Name
splicing factor 3b subunit 4
Uniprot IDQ15427
Protein Name
Splicing factor 3B subunit 4
Alternate Names
AFD1 • Hsh49 • pre-mRNA-splicing factor SF3b 49 kDa subunit • SAP 49 • SAP49 • SF3b49 • SF3b50 • spliceosomal protein • spliceosome-associated protein (U2 snRNP) • spliceosome-associated protein 49 • splicing factor 3B subunit 4 • splicing factor 3b, subunit 4, 49kD • splicing factor 3b, subunit 4, 49kDa
Product Information
Applications
IHC, IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to SF3B4 immobilized on solid support.
The epitope recognized by A303-950A maps to a region between residue 374 to 424 of human Splicing Factor 3b, Subunit 4, 49kDa using the numbering given in entry NP_005841.1 (GeneID 10262).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:500 - 1:2000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A303-950A · Lot 1 (current lot)Open
Product DatasheetPDF · A303-950A · Lot 1 (current lot)Open
Safety Data SheetPDF · A303-950AOpen
Citations / Publications
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