Bethyl Laboratories

Rabbit anti-SFRS14 IHC Antibody Affinity Purified

Primary
Catalog #IHC-00318
TargetSFRS14
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, IHC-IF
Detection of human SFRS14 by immunohistochemistry.
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Product Description

SFRS14 (splicing factor arginine/serine-rich 14) is a putative alternative splicing factor. It contains two SURP motif repeats which are found among proteins involved in regulated mRNA splicing and a G-patch domain which features six highly conserved glycine residues found in several RNA-associated proteins. SFRS14 also bears an N-terminal arginine/serine-rich region found among the SR-related family of pre-mRNA processing factors.

Specifications

Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 725 and 775
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
50 µg/ml
Target Identity
TargetSFRS14
Antigen Species
Human
Gene ID10147
Gene Symbol
SUGP2
Gene Name
SURP and G-patch domain containing 2
Uniprot IDQ8IX01
Protein Name
SURP and G-patch domain-containing protein 2
Product Information
Applications
IHC, IHC-IF
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA

Production & Epitope

Antibody was affinity purified using an epitope specific to SFRS14 immobilized on solid support.

The epitope recognized by IHC-00318 maps to a region between residue 725 and 775 of human splicing factor, arginine/serine-rich 14 using the numbering given in entry NP_055699.1 (GeneID 10147).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Applications

Epitope exposure is recommended.

Epitope exposure with citrate buffer will enhance staining.

Likely to work with frozen sections.

In some cases, the antibody may be diluted further than indicated.

Immunohistochemistry (IHC)1:100 - 1:500
Immunohistochemistry-Immunofluoresence (IHC-IF)1:50 - 1:500

Documents

Safety Data SheetPDF · IHC-00318Open
Product DatasheetPDF · IHC-00318 · Lot 1 (current lot)Open
Product DatasheetPDF · IHC-00318 · Lot 1 (current lot)Open
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