Bethyl Laboratories
Rabbit anti-SFRS18/SRrp130 Antibody Affinity Purified
Primary
Catalog #A301-609A
TargetSFRS18/SRrp130
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP
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Product Description
SFRS18/SRrp130 (splicing factor arginine/serine-rich 18) is a putative alternative splicing factor. It was identified as a factor that interacts with pinin (Pnn/DRS/memA), a cell-adhesion and nuclear protein of corneal epithelial cells. SFRS18/SRrp130 bears multiple arginine/serine-rich repeats found among the SR-related family of pre-mRNA processing factors. SFRS18/SRrp130 and pinin co-localize and are distributed as nuclear specks that correspond to the pre-splicing machinery.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 200 and 250
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetSFRS18/SRrp130
Antigen Species
Human
Gene ID25957
Gene Symbol
PNISR
Gene Name
PNN interacting serine and arginine rich protein
Uniprot IDQ8TF01
Protein Name
Arginine/serine-rich protein PNISR
Alternate Names
arginine/serine-rich protein PNISR • bA98I9.2 • C6orf111 • HSPC306 • PNN-interacting serine/arginine-rich protein • Serine/arginine-rich-splicing regulatory protein 130 • serine-arginine-rich-splicing regulatory protein 130 • SFRS18 • splicing factor, arginine/serine-rich 130 • splicing factor, arginine/serine-rich 18 • SR-related protein • SR-rich protein • SRrp130
Product Information
Applications
IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to SFRS18/SRrp130 immobilized on solid support.
The epitope recognized by A301-609A maps to a region between residue 200 to 250 of human splicing factor, arginine/serine-rich 18 (splicing factor, arginine/serine-rich 130) using the numbering given in entry NP_056306.1 (GeneID 25957).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A301-609A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-609A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-609AOpen
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