Bethyl Laboratories
Rabbit anti-SIL Antibody Affinity Purified
Primary
Catalog #A302-442A
TargetSIL
HostRabbit
ClonalityPolyclonal
ApplicationIP, WB
Image 1 of 3
local_shipping
Order within 14 hours and 52 minutes for shipment by Tuesday, November 17
Product Description
SCL-interrupting locus (SIL) plays an important role in embryonic development as well as in cellular growth and proliferation. The long-term silencing of SIL affects cell survival and cell cycle distribution as well as decreases CDC2 activity correlated with reduced phosphorylation of CDC2. SIL is a positive regulator of the sonic hedgehog pathway, acting downstream of PTCH1. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q15468].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1237 and C-terminus
Isotype
IgG
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetSIL
Antigen Species
Human
Gene ID6491
Gene Symbol
STIL
Gene Name
SCL/TAL1 interrupting locus
Uniprot IDQ15468
Protein Name
SCL-interrupting locus protein
Alternate Names
Product Information
Applications
IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to SIL immobilized on solid support.
The epitope recognized by A302-442A maps to a region between residue 1237 and 1287 of human SCL/TAL1 interrupting locus using the numbering given in entry NP_003026.2 (GeneID 6491).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A302-442A · Lot 2 (current lot)Open
Product DatasheetPDF · A302-442A · Lot 2 (current lot)Open
Safety Data SheetPDF · A302-442AOpen
Citations / Publications
Services
Antibody Conjugation
Antibody and conjugate formats developed together, built to your specification.
Request CustomizationCustom Monoclonal Antibody Discovery Services
Full custom monoclonal development, from immunization through validation.
Explore Monoclonal DevelopmentVHH Discovery
Single-domain antibodies for structurally difficult targets a conventional monoclonal can't reach.
Explore VHH DiscoveryResources
1