Bethyl Laboratories
Rabbit anti-SIX4 Antibody Affinity Purified
Primary
Catalog #A303-219A
TargetSIX4
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP
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Product Description
SIX4 SIX homeobox 4 is a member of the homeobox family, subfamily SIX. The drosophila homolog is a nuclear homeoprotein required for eye development. Studies in mouse show that this gene product functions as a transcription factor, and may have a role in the differentiation or maturation of neuronal cells [taken from NCBI Entrez Gene (Gene ID: 51804)].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 550 and 600
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetSIX4
Antigen Species
Human
Gene ID51804
Gene Symbol
SIX4
Gene Name
SIX homeobox 4
Uniprot IDQ9UIU6
Protein Name
Homeobox protein SIX4
Alternate Names
Product Information
Applications
IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to SIX4 immobilized on solid support.
The epitope recognized by A303-219A maps to a region between residue 550 and 600 of human Sine Oculis Homeobox Homolog 4 using the numbering given in entry NP_059116.3 (GeneID 51804).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A303-219A · Lot 1 (current lot)Open
Product DatasheetPDF · A303-219A · Lot 1 (current lot)Open
Safety Data SheetPDF · A303-219AOpen
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