Bethyl Laboratories
Rabbit anti-SMAD3 Recombinant Monoclonal Antibody [BLR144J]
Monoclonal Antibodies
Catalog #A700-144
TargetSMAD3
HostRabbit
CloneBLR144J
ReactivityHuman
ApplicationIP, WB
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Product Description
The SMAD family of proteins are a group of intracellular signal transducer proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. The SMAD3 protein functions in the transforming growth factor-beta signaling pathway, and transmits signals from the cell surface to the nucleus, regulating gene activity and cell proliferation. It also functions as a tumor suppressor. [taken from NCBI Entrez Gene (Gene ID: 4088)].
Specifications
Clonality
Recombinant Monoclonal
Clone
BLR144J
Host
Rabbit
Immunogen
Between 200 and 250
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Purified
Concentration
400 µg/ml
Target Identity
TargetSMAD3
Antigen Species
Human
Gene ID4088
Gene Symbol
SMAD3
Gene Name
SMAD family member 3
Uniprot IDP84022
Protein Name
Mothers against decapentaplegic homolog 3
Alternate Names
hMAD-3 • hSMAD3 • HSPC193 • HsT17436 • JV15-2 • LDS1C • LDS3 • MAD homolog 3 • mad homolog JV15-2 • mad protein homolog • MAD, mothers against decapentaplegic homolog 3 • mad3 • MADH3 • mothers against decapentaplegic homolog 3 • mothers against DPP homolog 3 • SMA- and MAD-related protein 3 • SMAD 3 • SMAD family member 3 • SMAD, mothers against DPP homolog 3 • Smad3
Product Information
Applications
IP, WB
Buffer
Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Recombinant antibody was purified from cell culture supernatant.
Immunogen was a peptide representing a region between residue 200 and 250 of human Mothers against decapentaplegic homolog 3 (SMAD3) using the numbering given in entry NP_005893.1 (Gene ID 4088).
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)15 µl/mg lysate
Western Blot (WB)1:1000
Documents
Product DatasheetPDF · A700-144 · Lot 1 (current lot)Open
Product DatasheetPDF · A700-144 · Lot 1 (current lot)Open
Product DatasheetPDF · A700-144 · Lot 1 (current lot)Open
Safety Data SheetPDF · A700-144Open
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