Bethyl Laboratories
Rabbit anti-SMAD4 Recombinant Monoclonal Antibody [BLR133J]
Monoclonal Antibodies
Catalog #A700-133
TargetSMAD4
HostRabbit
CloneBLR133J
ReactivityMouse, Human
ApplicationFlow Cyt, ICC, IHC, IP, WB
Image 1 of 7
local_shipping
Order within 20 hours and 40 minutes for shipment by Tuesday, November 17
Product Description
The SMAD family of proteins are a group of intracellular signal transducer proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. SMAD4 forms homomeric complexes and heteromeric complexes with other activated Smad proteins, which then accumulate in the nucleus and regulate the transcription of target genes. SMAD4 functions in the transforming growth factor-beta signaling pathway as well as the bone morphogenetic protein signaling pathway, regulating cell proliferation, angiogenesis, and increasing blood vessel hyperpermeability. [taken from NCBI Entrez Gene (Gene ID: 4089)].
Specifications
Clonality
Recombinant Monoclonal
Clone
BLR133J
Host
Rabbit
Immunogen
Between 1 and 50
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Purified
Concentration
1000 µg/ml
Target Identity
TargetSMAD4
Antigen Species
Human
Gene ID4089
Gene Symbol
SMAD4
Gene Name
SMAD family member 4
Uniprot IDQ13485
Protein Name
Mothers against decapentaplegic homolog 4
Alternate Names
deleted in pancreatic carcinoma locus 4 • deletion target in pancreatic carcinoma 4 • DPC4 • hSMAD4 • JIP • MAD homolog 4 • MADH4 • mothers against decapentaplegic homolog 4 • mothers against decapentaplegic, Drosophila, homolog of, 4 • Mothers against DPP homolog 4 • MYHRS • SMAD 4 • SMAD family member 4 • SMAD, mothers against DPP homolog 4 • Smad4
Product Information
Applications
Flow Cyt, ICC, IHC, IP, WB
Buffer
Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Recombinant antibody was purified from cell culture supernatant.
Immunogen was a peptide representing a region between residue 1 and 50 of human mothers against decapentaplegic homolog 4 (SMAD4) using the numbering given in entry NP_005350.1 (Gene ID 4089).
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Flow Cytometry (Flow Cyt)Fixed in 4% formaldehyde and permeabilized with 90% methanol. 0.25 µl per 1 x 10^6 cells.
Immunocytochemistry (ICC)1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
Immunohistochemistry (IHC)1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µl/mg lysate
Western Blot (WB)1:1000
Documents
Product DatasheetPDF · A700-133 · Lot 1 (current lot)Open
Product DatasheetPDF · A700-133 · Lot 1 (current lot)Open
Safety Data SheetPDF · A700-133Open
Services
Antibody Conjugation
Antibody and conjugate formats developed together, built to your specification.
Request CustomizationCustom Monoclonal Antibody Discovery Services
Full custom monoclonal development, from immunization through validation.
Explore Monoclonal DevelopmentVHH Discovery
Single-domain antibodies for structurally difficult targets a conventional monoclonal can't reach.
Explore VHH DiscoveryResources
1