Bethyl Laboratories
Rabbit anti-SMG1 Antibody Affinity Purified
Primary
Catalog #A301-535A
TargetSMG1
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationWB
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Product Description
SMG1 is a PIKK (phosphoinositide 3-kinase related kinases) protein kinase that plays a key role in RNA surveillance via nonsense-mediated mRNA decay (NMD). SMG1 phosphorylates Upf1, a factor that possesses RNA helicase activity and is also central to NMD. SMG1 and UPF-1 have been shown to exist in a complex with the translation termination release factors eRF3, and eRF1. This complex has been termed SURF and associates with the exon junction complex (EJC) to function in the recognition of premature termination codons in aberrant RNAs to trigger NMD.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1250 and 1300
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetSMG1
Antigen Species
Human
Gene ID23049
Gene Symbol
SMG1
Gene Name
SMG1, nonsense mediated mRNA decay associated PI3K related kinase
Uniprot IDQ96Q15
Protein Name
Serine/threonine-protein kinase SMG1
Alternate Names
61E3.4 • ATX • hSMG-1 • lambda/iota protein kinase C-interacting protein • lambda-interacting protein • LIP • PI-3-kinase-related kinase SMG-1 • serine/threonine-protein kinase SMG1 • SMG-1 • smg-1 homolog, phosphatidylinositol 3-kinase-related kinase • SMG1 phosphatidylinositol 3-kinase-related kinase
Product Information
Applications
WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to SMG1 immobilized on solid support.
The epitope recognized by A301-535A maps to a region between residue 1250 and 1300 of human PI-3-Kinase-related kinase SMG1 using the numbering given in entry NP_055907.3 (GeneID 23049).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western Blot (WB)1:1000 - 1:10,000 (3-8% Tris-acetate gel recommended)
Documents
Product DatasheetPDF · A301-535A · Lot 2 (current lot)Open
Product DatasheetPDF · A301-535A · Lot 2 (current lot)Open
Safety Data SheetPDF · A301-535AOpen
Citations / Publications
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Resources
Standard Western Blot Protocol
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