Bethyl Laboratories
Rabbit anti-SMG7 Antibody Affinity Purified
Primary
Catalog #A302-170A
TargetSMG7
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIP, WB
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Product Description
SMG7 plays a role in the dephosphorylation of UPF1, an RNA-dependent ATPase and helicase critical to nonsense-mediated mRNA decay (NMD). It is a homolog of the C. elegans NMD factor Smg7 (suppressor with morphogenetic effect on genitalia protein 7). SMG7 functions as an adaptor to target protein phosphatase 2A to UPF1 for dephosphorylation of UPF1.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1087 and C-terminus
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetSMG7
Antigen Species
Human
Gene ID9887
Gene Symbol
SMG7
Gene Name
SMG7, nonsense mediated mRNA decay factor
Uniprot IDQ92540
Protein Name
Protein SMG7
Product Information
Applications
IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to SMG7 immobilized on solid support.
The epitope recognized by A302-170A maps to a region between residue 1087 and 1137 of human suppressor with morphogenetic effect on genitalia protein 7 (ever shorter telomeres 1C) using the numbering given in entry NP_775179.1 (GeneID 9887).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A302-170A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-170A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-170AOpen
Citations / Publications
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