Bethyl Laboratories
Rabbit anti-SMRT Antibody Affinity Purified
Primary
Catalog #A301-147A
TargetSMRT
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
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Product Description
SMRT (silencing mediator of retinoid and thyroid hormone receptor) mediates gene repression by interacting with nuclear receptors such as the thyroid hormone receptor, retinoic acid receptor, progesterone receptor, androgen receptor, estrogen receptor, and glucocorticoids receptor. SMRT mediates repression by associating with SIN3A/B and the histone deacetylases, HDAC1 and HDAC2, and promoting the formation of repressive chromatin structures.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1525 and 1575
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetSMRT
Antigen Species
Human
Gene ID9612
Gene Symbol
NCOR2
Gene Name
nuclear receptor corepressor 2
Uniprot IDQ9Y618
Protein Name
Nuclear receptor corepressor 2
Alternate Names
CTG repeat protein 26 • CTG26 • N-CoR2 • nuclear receptor corepressor 2 • silencing mediator for retinoid and thyroid hormone receptors • Silencing mediator of retinoic acid and thyroid hormone receptor • SMAP270 • SMRT • SMRTE • SMRTE-tau • T3 receptor-associating factor • thyroid-, retinoic-acid-receptor-associated corepressor • TNRC14 • TRAC • TRAC1 • TRAC-1
Product Information
Applications
IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to SMRT immobilized on solid support.
The epitope recognized by A301-147A maps to a region between residue 1525 and 1575 of human silencing mediator of retinoic acid and thyroid hormone receptor alpha using the numbering given in entry AAD20946.1 (GeneID 9612).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000 (3-8% Tris-acetate gel recommended)
Documents
Product DatasheetPDF · A301-147A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-147A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-147AOpen
Citations / Publications
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