Bethyl Laboratories
Rabbit anti-SOX10 Antibody Affinity Purified
Primary
Catalog #A305-799A
TargetSOX10
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationICC, IHC, IHC-IF, IP, WB
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Product Description
SOX10 is a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. SOX10 may act as a transcriptional activator after forming a protein complex with other proteins. SOX10 acts as a nucleocytoplasmic shuttle protein and is important for neural crest and peripheral nervous system development [taken from NCBI Entrez Gene (Gene ID: 6663)].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 416 and C-terminus
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetSOX10
Antigen Species
Human
Gene ID6663
Gene Symbol
SOX10
Gene Name
SRY-box 10
Uniprot IDP56693
Protein Name
Transcription factor SOX-10
Alternate Names
Product Information
Applications
ICC, IHC, IHC-IF, IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to SOX10 immobilized on solid support. The epitope recognized by A305-799A maps to a region between residue 416 and 466 of human DOM; WS4; PCWH; WS2E; WS4C using the numbering given in entry P56693.1 (GeneID 6663).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunocytochemistry (ICC)1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
Immunohistochemistry (IHC)1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Immunohistochemistry-Immunofluoresence (IHC-IF)1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:10,000 - 1:25,000
Documents
Product DatasheetPDF · A305-799A · Lot 2 (current lot)Open
Product DatasheetPDF · A305-799A · Lot 2 (current lot)Open
Safety Data SheetPDF · A305-799AOpen
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