Bethyl Laboratories
Rabbit anti-SPAK Antibody Affinity Purified
Primary
Catalog #A302-466A
TargetSPAK
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, WB
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Product Description
STE20/SPS1-related proline-alanine rich protein kinase (SPAK) is a serine/threonine kinase that is thought to function in the cellular stress response pathway. This kinase is activated in response to hypotonic stress, leading to phosphorylation of several cation-chloride-coupled cotransporters. Catalytically active SPAK specifically activates the p38 MAP kinase pathway, and its interaction with p38 decreases upon cellular stress, suggesting that this kinase may serve as an intermediate in the response to cellular stress [taken from NCBI Entrez Gene (GeneID: 27347)].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 425 and 475
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetSPAK
Antigen Species
Human
Gene ID27347
Gene Symbol
STK39
Gene Name
serine/threonine kinase 39
Uniprot IDQ9UEW8
Protein Name
STE20/SPS1-related proline-alanine-rich protein kinase
Alternate Names
DCHT • PASK • proline-alanine-rich STE20-related kinase • serine threonine kinase 39 (STE20/SPS1 homolog, yeast) • serine/threonine-protein kinase 39 • small intestine SPAK-like kinase • SPAK • STE20/SPS1 homolog • STE20/SPS1-related proline-alanine-rich protein kinase • Ste20-like protein kinase • ste-20-related kinase
Product Information
Applications
IHC, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to SPAK immobilized on solid support.
The epitope recognized by A302-466A maps to a region between residue 425 and 450 of human STE20/SPS1-related proline-alanine-rich protein kinase using the numbering given in entry AAC72238.1 (GeneID 27347).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Immunohistochemistry (IHC)1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A302-466A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-466A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-466AOpen
Citations / Publications
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