Bethyl Laboratories
Rabbit anti-SPHK1 Antibody Affinity Purified
Primary
Catalog #A302-176A
TargetSPHK1
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP
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Product Description
Sphingosine kinase 1 (SPHK1) catalyzes the phosphorylation of sphingosine to yield sphingosine-1-phosphate (SPP). SPP functions as a lipid signaling molecule that mediates both intra- and extra-cellular responses. SPHK1activity may play a role in several cellular processes such as apoptisis, proliferation, inflammation, and migration.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1 and 50
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetSPHK1
Antigen Species
Human
Gene ID8877
Gene Symbol
SPHK1
Gene Name
sphingosine kinase 1
Uniprot IDQ9NYA1
Protein Name
Sphingosine kinase 1
Alternate Names
SK 1 • sphingosine kinase 1 • SPHK • SPK 1
Product Information
Applications
IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to SPHK1 immobilized on solid support.
The epitope recognized by A302-176A maps to a region between residue 1 and 50 of human sphingosine kinase 1 using the numbering given in entry NP_001136073.1 (GeneID 8877).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A302-176A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-176A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-176AOpen
Citations / Publications
Services
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