/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-SPINDLY/CCDC99 Antibody
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Primary Antibodies  

Rabbit anti-SPINDLY/CCDC99 Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Target:

SPINDLY/CCDC99

Reactivity:

Human

Applications:

IP

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 555 and C-terminus

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Production Procedures

Antibody was affinity purified using an epitope specific to SPINDLY/CCDC99 immobilized on solid support.

The epitope recognized by A301-355A-T maps to a region between residue 525 and 575 of human SPINDLY (coiled-coil domain containing 99) using the numbering given in entry NP_060255.2 (GeneID 54908).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Antibody was affinity purified using an epitope specific to SPINDLY/CCDC99 immobilized on solid support.

The epitope recognized by A301-355A maps to a region between residue 525 and 575 of human SPINDLY (coiled-coil domain containing 99) using the numbering given in entry NP_060255.2 (GeneID 54908).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Additional Product Information

Spindly has recently been identified as the human homolog of Drosophila spindly. Spindly has been shown to regulate mitotic dynein and is essential for the docking and recruitment of dynein to the kinetochore.

54908

SPDL1

spindle apparatus coiled-coil protein 1

Q96EA4

Protein Spindly

Alternate Names

arsenite-related gene 1 protein; CCDC99; coiled-coil domain-containing protein 99; hSpindly; protein Spindly; rhabdomyosarcoma antigen MU-RMS-40.4A; rrhabdomyosarcoma antigen protein MU-RMS-40.4A; Spindle apparatus coiled-coil domain-containing protein 1

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.