Bethyl Laboratories
Rabbit anti-SPINDLY/CCDC99 Antibody Affinity Purified
Primary
Catalog #A301-355A
TargetSPINDLY/CCDC99
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP
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Product Description
Spindly has recently been identified as the human homolog of Drosophila spindly. Spindly has been shown to regulate mitotic dynein and is essential for the docking and recruitment of dynein to the kinetochore.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 555 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetSPINDLY/CCDC99
Antigen Species
Human
Gene ID54908
Gene Symbol
SPDL1
Gene Name
spindle apparatus coiled-coil protein 1
Uniprot IDQ96EA4
Protein Name
Protein Spindly
Product Information
Applications
IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to SPINDLY/CCDC99 immobilized on solid support.
The epitope recognized by A301-355A maps to a region between residue 525 and 575 of human SPINDLY (coiled-coil domain containing 99) using the numbering given in entry NP_060255.2 (GeneID 54908).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A301-355A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-355A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-355AOpen
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