Bethyl Laboratories
Rabbit anti-SPTAN1/Alpha II-spectrin Antibody Affinity Purified
Primary
Catalog #A301-249A
TargetSPTAN1/Alpha II-spectrin
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC, IHC-IF, WB
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Product Description
SPTAN1/Alpha II-spectrin is one of two polypeptide chains (alpha and beta) that are part of the fodrin tetramer. Fodrin functions to link actin filaments to the plasma membrane and is a major neuronal cytoskeletal protein. Fodrin has been linked to neurogenerative and autoimmune diseases.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 2422 and C-terminus
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetSPTAN1/Alpha II-spectrin
Antigen Species
Human
Gene ID6709
Gene Symbol
SPTAN1
Gene Name
spectrin alpha, non-erythrocytic 1
Uniprot IDQ13813
Protein Name
Spectrin alpha chain, non-erythrocytic 1
Product Information
Applications
IHC, IHC-IF, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to SPTAN1/Alpha II-spectrin immobilized on solid support.
The epitope recognized by A301-249A maps to a region between residue 2422 and 2472 of human spectrin, alpha, non-erythrocytic 1 using the numbering given in entry NP_003118.1 (GeneID 6709).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Immunohistochemistry (IHC)1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunohistochemistry-Immunofluoresence (IHC-IF)1:50 - 1:250
Western Blot (WB)1:2000 - 1:10,000 (3-8% Tris-acetate gel recommended)
Documents
Product DatasheetPDF · A301-249A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-249A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-249AOpen
Citations / Publications
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