Bethyl Laboratories
Rabbit anti-SRBC Antibody Affinity Purified
Primary
Catalog #A302-419A
TargetSRBC
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
Image 1 of 1
local_shipping
Order within 19 hours and 41 minutes for shipment by Tuesday, November 17
Product Description
Serum deprivation response factor-related gene product that binds to C-kinase (SRBC) was identified as a binding protein of the protein kinase C, delta (PRKCD). The expression of this gene in cultured cell lines is strongly induced by serum starvation. The expression of this protein was found to be down-regulated in various cancer cell lines, suggesting the possible tumor suppressor function of this protein [taken from NCBI Entrez Gene (GeneID:112464)].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 211 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetSRBC
Antigen Species
Human
Gene ID112464
Gene Symbol
CAVIN3
Gene Name
protein kinase C delta binding protein
Uniprot IDQ969G5
Protein Name
Protein kinase C delta-binding protein
Product Information
Applications
IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to SRBC immobilized on solid support.
The epitope recognized by A302-419A maps to a region between residue 250 and 261 of human sdr-related gene product that binds to c-kinase using the numbering given in entry NP_659477.2 (GeneID 112464).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A302-419A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-419A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-419AOpen
Citations / Publications
Services
Antibody Conjugation
Antibody and conjugate formats developed together, built to your specification.
Request CustomizationCustom Monoclonal Antibody Discovery Services
Full custom monoclonal development, from immunization through validation.
Explore Monoclonal DevelopmentVHH Discovery
Single-domain antibodies for structurally difficult targets a conventional monoclonal can't reach.
Explore VHH DiscoveryResources
1