Bethyl Laboratories
Rabbit anti-STAT5a Recombinant Monoclonal Antibody [BLR124H]
Monoclonal Antibodies
Catalog #A700-124
TargetSTAT5a
HostRabbit
CloneBLR124H
ReactivityMouse, Human
ApplicationFlow Cyt, ICC, IHC, IP, WB
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Product Description
Signal transducer and activator of transcription 5a (STAT5a) is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones [taken from NCBI Entrez Gene (Gene ID: 6776)].
Specifications
Clonality
Recombinant Monoclonal
Clone
BLR124H
Host
Rabbit
Immunogen
Between 744 and C-terminus
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Purified
Concentration
100 µg/ml
Target Identity
TargetSTAT5a
Antigen Species
Human
Gene ID6776
Gene Symbol
STAT5A
Gene Name
signal transducer and activator of transcription 5A
Uniprot IDP42229
Protein Name
Signal transducer and activator of transcription 5A
Product Information
Applications
Flow Cyt, ICC, IHC, IP, WB
Buffer
Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Recombinant antibody was purified from cell culture supernatant.
Immunogen was a peptide representing a region between residue 744 and the C-terminus (residue 794) of human signal transducer and activator of transcription 5a using the numbering given in entry NP_001275647.1 (Gene ID 6776).
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Flow Cytometry (Flow Cyt)Fixed in 4% formaldehyde and permeabilized with 90% methanol. 2 µl per 1 x 10^6 cells.
Immunocytochemistry (ICC)1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
Immunohistochemistry (IHC)1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)20 µl/mg lysate
Western Blot (WB)1:1000
Documents
Product DatasheetPDF · A700-124 · Lot 1 (current lot)Open
Product DatasheetPDF · A700-124 · Lot 1 (current lot)Open
Product DatasheetPDF · A700-124 · Lot 1 (current lot)Open
Safety Data SheetPDF · A700-124Open
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Resources
Standard Western Blot Protocol
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Immunoprecipitation Protocol
Read the Protocol
Simple Western™ Resources
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