Bethyl Laboratories
Rabbit anti-STIM2 Antibody Affinity Purified
Primary
Catalog #A304-429A
TargetSTIM2
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIP, WB
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Product Description
STIM2 (stromal interaction molecule 2) plays a role in mediating store-operated Ca(2+) entry (SOCE), a Ca(2+) influx following depletion of intracellular Ca(2+) stores. Stim2 functions as a highly sensitive Ca(2+) sensor in the endoplasmic reticulum which activates both store-operated and store-independent Ca(2+)-influx. It regulates basal cytosolic and endoplasmic reticulum Ca(2+) concentrations. Upon mild variations of the endoplasmic reticulum Ca(2+) concentration, STIM2 translocates from the endoplasmic reticulum to the plasma membrane where it probably activates the Ca(2+) release-activated Ca(2+) (CRAC) channels ORAI1, ORAI2 and ORAI. STIM2 may inhibit STIM1-mediated Ca(2+) influx [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9P246].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 600 and 650
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetSTIM2
Antigen Species
Human
Gene ID57620
Gene Symbol
STIM2
Gene Name
stromal interaction molecule 2
Uniprot IDQ9P246
Protein Name
Stromal interaction molecule 2
Alternate Names
Product Information
Applications
IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to STIM2 immobilized on solid support.
The epitope recognized by A304-429A maps to a region between residue 600 to 650 of human Stromal Interaction Molecule 2 using the numbering given in entry NP_065911.3 (GeneID 57620).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A304-429A · Lot 1 (current lot)Open
Product DatasheetPDF · A304-429A · Lot 1 (current lot)Open
Safety Data SheetPDF · A304-429AOpen
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