Bethyl Laboratories
Rabbit anti-Symplekin Antibody Affinity Purified
Primary
Catalog #A301-464A
TargetSymplekin
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
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Product Description
Symplekin is a component of tight junctions that also localizes to the nucleus and is a component of 3’-end processing machinery. As part of the mRNA 3’-end processing machinery symplekin is necessary for poly(A) tail elongation. Although histone mRNA 3’ends are not polyadenylated, symplekin has been shown to also play a role in 3’end maturation of histone mRNAs.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 350 and 400
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetSymplekin
Antigen Species
Human
Gene ID8189
Gene Symbol
SYMPK
Gene Name
symplekin
Uniprot IDQ92797
Protein Name
Symplekin
Product Information
Applications
IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to Symplekin immobilized on solid support.
The epitope recognized by A301-464A maps to a region between residue 350 and 400 of human Symplekin using the numbering given in entry NP_004810.2 (GeneID 8189).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:10,000 - 1:25,000
Documents
Product DatasheetPDF · A301-464A · Lot 2 (current lot)Open
Product DatasheetPDF · A301-464A · Lot 2 (current lot)Open
Safety Data SheetPDF · A301-464AOpen
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