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Rabbit anti-Symplekin IHC Antibody

Bethyl Laboratories®

Bethyl Laboratories® Catalog #

Validated
Target:
Symplekin
Reactivity:
Human
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 1224 and 1274
IsoType:
IgG
Purity:
Antigen Affinity Purified
100 µl (50+ slides)

10 µl (5+ slides)

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Product Details

Specifications
Verified Reactivity
Human
Presumed Reactivity
Monkey, Gorilla, Chimpanzee, Orangutan
Antigen Species
Human
Stock Concentration
250 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
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Prod Procedures
Antibody was affinity purified using an epitope specific to Symplekin immobilized on solid support.

The epitope recognized by IHC-00531-T maps to a region between residue 1224 and 1274 of human Symplekin using the numbering given in entry NP_004810.2 (GeneID 8189).

Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

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Gene & Protein Information

Symplekin is a component of tight junctions that also localizes to the nucleus and is a component of 3’-end processing machinery. As part of the mRNA 3’-end processing machinery symplekin is necessary for poly(A) tail elongation. Although histone mRNA 3’ends are not polyadenylated, symplekin has been shown to also play a role in 3’end maturation of histone mRNAs.

8189

SYMPK

symplekin

Q92797

Symplekin

Alternate Names

SPK, SYM, symplekin

Applications

Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining. Likely to work with frozen sections. In some cases, the antibody may be diluted further than indicated.