Bethyl Laboratories
Rabbit anti-Synoviolin Antibody Affinity Purified
Primary
Catalog #A302-945A
TargetSynoviolin
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP
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Product Description
The E3 ubiquitin-protein ligase synoviolin is also known as human HRD1. The original HRD1 (HMG-CoA reductase degradation) gene was identified as a mutant gene in yeast deficient for the degradation of 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR). Homology between the human and yeast gene is limited to the N-terminal domain of the proteins, and human HRD1/Synoviolin does not appear to be involved in the degradation of mammalian HMGR. Synoviolin is involved in endoplasmic reticulum (ER)-associated degradation. It removes unfolded proteins, accumulated during ER stress, by retrograde transport to the cytosol from the ER. This protein also uses the ubiquitin-proteasome system for additional degradation of unfolded proteins.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 567 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetSynoviolin
Antigen Species
Human
Gene ID84447
Gene Symbol
SYVN1
Gene Name
synoviolin 1
Uniprot IDQ86TM6
Protein Name
E3 ubiquitin-protein ligase synoviolin
Product Information
Applications
IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to Synoviolin immobilized on solid support.
The epitope recognized by A302-945A maps to a region between residue 550 and 600 of human Synovial Apoptosis Inhibitor 1 using the numbering given in entry NP_757385.1 (GeneID 84447).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A302-945A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-945A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-945AOpen
Services
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