Bethyl Laboratories
Rabbit anti-TAB182 Antibody Affinity Purified
Primary
Catalog #A301-438A
TargetTAB182
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
Image 1 of 1
local_shipping
Order within 16 hours and 26 minutes for shipment by Tuesday, November 17
Product Description
TAB182 (tankyrase 1-binding protein of 182 kDa) was identified in a two-hybrid screen in search of tankyrase 1-interacting proteins. Tankyrase 1 is a poly (ADP-ribose) polymerase (PARP) that ribosylates the negative regulator of telomere length, TRF1 (telomere-repeat binding factor). Ribosylation of TRF1 has been shown to result in the inhibition of TRF1 binding and an induction of telomere elongation. TAB182 and TRF1 bind multiple discrete and overlapping sites of the tankyrase 1 ankyrin domain. This finding suggests that TAB182 may act as a scaffold to mediate higher order protein complex formation at telomeres. TAB182 has also been suggested to serve a role in the cytoplasm as it has been found to localize to the cytoplasmic cortical actin network.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 350 and 400
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetTAB182
Antigen Species
Human
Gene ID85456
Gene Symbol
TNKS1BP1
Gene Name
tankyrase 1 binding protein 1
Uniprot IDQ9C0C2
Protein Name
182 kDa tankyrase-1-binding protein
Product Information
Applications
IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to TAB182 immobilized on solid support.
The epitope recognized by A301-438A maps to a region between residue 350 and 400 of human tankyrase 1-binding protein of 182 kDa using the numbering given in entry NP_203754.2 (GeneID 85456).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A301-438A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-438A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-438AOpen
Services
Antibody Conjugation
Antibody and conjugate formats developed together, built to your specification.
Request CustomizationCustom Monoclonal Antibody Discovery Services
Full custom monoclonal development, from immunization through validation.
Explore Monoclonal DevelopmentVHH Discovery
Single-domain antibodies for structurally difficult targets a conventional monoclonal can't reach.
Explore VHH DiscoveryResources
1