Bethyl Laboratories
Rabbit anti-TBRG4/CPR2 Antibody Affinity Purified
Primary
Catalog #A301-392A
TargetTBRG4/CPR2
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
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Product Description
TBRG4 (transforming growth factor beta regulator 4) was originally identified as a cell cycle progression restoration (CPR) gene that, when overexpressed, was capable of promoting G1 progression in the presence of yeast pheromone arresting signals.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 50 and 100
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetTBRG4/CPR2
Antigen Species
Human
Gene ID9238
Gene Symbol
TBRG4
Gene Name
transforming growth factor beta regulator 4
Uniprot IDQ969Z0
Protein Name
Protein TBRG4
Product Information
Applications
IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to TBRG4/CPR2 immobilized on solid support.
The epitope recognized by A301-392A maps to a region between residue 25 and 75 of human transforming growth factor beta regulator 4 (cell cycle progression restoration 2) using the numbering given in entry NP_004740.2 (GeneID 9238).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A301-392A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-392A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-392AOpen
Citations / Publications
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