Bethyl Laboratories
Rabbit anti-TEIF Antibody Affinity Purified
Primary
Catalog #A302-653A
TargetTEIF
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIP, WB
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Product Description
TEIF (telomerase transcriptional element-interacting factor) belongs to the SCY1-like family of kinase-like proteins. The protein has a divergent N-terminal kinase domain that is thought to be catalytically inactive, and can bind specific DNA sequences through its C-terminal domain. It activates transcription of the telomerase reverse transcriptase and DNA polymerase beta genes [taken from NCBI Entrez Gene (GeneID: 57410)].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 758 and C-terminus
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetTEIF
Antigen Species
Human
Gene ID57410
Gene Symbol
SCYL1
Gene Name
SCY1 like pseudokinase 1
Uniprot IDQ96KG9
Protein Name
N-terminal kinase-like protein
Alternate Names
coated vesicle-associated kinase of 90 kDa • GKLP • HT019 • likely ortholog of mouse N-terminal kinase-like protein • NKTL • N-terminal kinase-like protein • NTKL • P105 • SCAR21 • SCY1-like protein 1 • SCY1-like, kinase-like 1 • TAPK • TEIF • telomerase regulation-associated protein • Telomerase transcriptional element-interacting factor • telomerase transcriptional elements-interacting factor • teratoma-associated tyrosine kinase • TRAP
Product Information
Applications
IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to TEIF immobilized on solid support.
The epitope recognized by A302-653A maps to a region between residue 758 and 808 of human telomerase transcriptional elements-interacting factor using the numbering given in entry NP_065731.3 (GeneID 57410).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A302-653A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-653A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-653AOpen
Citations / Publications
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