/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-TFEB Antibody
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Primary Antibodies  

Rabbit anti-TFEB Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Citations (195)
Target:

TFEB

Reactivity:

Human

,

Mouse

Applications:

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 426 and C-terminus

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

$121.00 $433.00
Qty:
Bulk Request

Product Details

Specifications
Verified Reactivity

Mouse,

Human

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Production Procedures

Antibody was affinity purified using an epitope specific to TFEB immobilized on solid support.

The epitope recognized by A303-673A maps to a region between residue 426 and 476 of human Transcription Factor EB using the numbering given in entry NP_009093.1 (GeneID 7942).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Country of Origin

USA

Additional Product Information

Transcription factor EB (TFEB) specifically recognizes and binds E-box sequences (3'-CANNTG-5'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFE3 or MITF. In association with TFE3, TFEB activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4+ T-cells and thymus-dependent humoral immunity. TFEB specifically recognizes and binds the CLEAR-box sequence (5'-GTCACGTGAC-3') present in the regulatory region of many lysosomal genes, leading to activate their expression; thus, TFEB plays a central role in expression of lysosomal genes. TFEB also specifically recognizes the gamma-E3 box, a subset of E-boxes, present in the heavy-chain immunoglobulin enhancer [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ P19484].

7942

TFEB

transcription factor EB

P19484

Transcription factor EB

Alternate Names

ALPHATFEB; BHLHE35; class E basic helix-loop-helix protein 35; T-cell transcription factor EB; TCFEB; transcription factor EB

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.