Bethyl Laboratories
Rabbit anti-TFIIS Antibody Affinity Purified
Primary
Catalog #A302-240A
TargetTFIIS
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC, WB
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Product Description
Transcription elongation factor S-II protein 2 (TFIIS) is necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q15560].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 200 and 250
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetTFIIS
Antigen Species
Human
Gene ID6917
Gene Symbol
TCEA1
Gene Name
transcription elongation factor A1
Uniprot IDP23193
Protein Name
Transcription elongation factor A protein 1
Alternate Names
Product Information
Applications
IHC, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to TFIIS immobilized on solid support.
The epitope recognized by A302-240A maps to a region between residue 200 and 250 of human transcription elongation factor S-II (transcription elongation factor A (SII), 1) using the numbering given in entry NP_006747.1 (GeneID 6917).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Immunohistochemistry (IHC)1:200 - 1:1000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A302-240A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-240A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-240AOpen
Citations / Publications
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