Bethyl Laboratories
Rabbit anti-TFIIS IHC Antibody Affinity Purified
Primary
Catalog #IHC-00656
TargetTFIIS
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC
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Product Description
Transcription elongation factor S-II protein 2 (TFIIS) is necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q15560].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1 and 50
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
100 µg/ml
Target Identity
TargetTFIIS
Antigen Species
Human
Gene ID6917
Gene Symbol
TCEA1
Gene Name
transcription elongation factor A1
Uniprot IDP23193
Protein Name
Transcription elongation factor A protein 1
Alternate Names
Product Information
Applications
IHC
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to TFIIS immobilized on solid support.
The epitope recognized by IHC-00656 maps to a region between residue 1 and 50 of human transcription elongation factor S-II (transcription elongation factor A (SII), 1) using the numbering given in entry NP_006747.1 (GeneID 6917).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
Epitope exposure is recommended.
Epitope exposure with citrate buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.
Immunohistochemistry (IHC)1:100 - 1:500
Documents
Safety Data SheetPDF · IHC-00656Open
Product DatasheetPDF · IHC-00656 · Lot 1 (current lot)Open
Product DatasheetPDF · IHC-00656 · Lot 1 (current lot)Open
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