Bethyl Laboratories
Rabbit anti-THOC5 Antibody Affinity Purified
Primary
Catalog #A302-119A
TargetTHOC5
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC, IP, WB
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Product Description
THOC5 is part of the splicesome complex. It has been identified as a target in macrophage-colony stimulating factor signaling and may have a potential role in leukemogenesis.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 25 and 75
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetTHOC5
Antigen Species
Human
Gene ID8563
Gene Symbol
THOC5
Gene Name
THO complex 5
Uniprot IDQ13769
Protein Name
THO complex subunit 5 homolog
Product Information
Applications
IHC, IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to THOC5 immobilized on solid support.
The epitope recognized by A302-119A maps to a region between residue 25 and 75 of human THO complex 5 using the numbering given in entry NP_003669.4 (GeneID 8563).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A302-119A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-119A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-119AOpen
Citations / Publications
Services
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