Bethyl Laboratories
Rabbit anti-TIGAR IHC Antibody Affinity Purified
Primary
Catalog #IHC-00651
TargetTIGAR
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC
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Product Description
TIGAR (TP53-induced glycolysis and apoptosis regulator) is a protein with sequence similarity to the bisphosphate domain of the glycolytic enzyme that degrades fructose-2,6-bisphosphate. The protein functions by blocking glycolysis and directing the pathway into the pentose phosphate shunt. Expression of this protein also protects cells from DNA damaging reactive oxygen species and provides some protection from DNA damage-induced apoptosis [taken from NCBI GeneID:57103].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 220 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
250 µg/ml
Target Identity
TargetTIGAR
Antigen Species
Human
Gene ID57103
Gene Symbol
TIGAR
Gene Name
TP53 induced glycolysis regulatory phosphatase
Uniprot IDQ9NQ88
Protein Name
Fructose-2,6-bisphosphatase TIGAR
Product Information
Applications
IHC
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to TIGAR immobilized on solid support.
The epitope recognized by IHC-00651 maps to a region between residue 220 and 270 of human TP53-induced glycolysis and apoptosis regulator using the numbering given in entry NP_065108.1 (GeneID 57103).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
Epitope exposure is recommended.
Epitope exposure with citrate buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.
Immunohistochemistry (IHC)1:100 - 1:500
Documents
Safety Data SheetPDF · IHC-00651Open
Product DatasheetPDF · IHC-00651 · Lot 1 (current lot)Open
Product DatasheetPDF · IHC-00651 · Lot 1 (current lot)Open
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