Bethyl Laboratories
Rabbit anti-TIGIT Recombinant Monoclonal Antibody [BLR047F]
Monoclonal Antibodies
Catalog #A700-047
TargetTIGIT
HostRabbit
CloneBLR047F
ReactivityHuman
ApplicationFlow Cyt, IHC, IHC-IF, WB
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Product Description
TIGIT is a member of the PVR (poliovirus receptor) family of immunoglobin proteins and is expressed on several classes of T cells including follicular B helper T cells (TFH). TIGIT has been shown to bind PVR with high affinity; this binding is thought to assist interactions between TFH and dendritic cells to regulate T cell dependent B cell responses. [taken from NCBI Entrez Gene (Gene ID: 201633)].
Specifications
Clonality
Recombinant Monoclonal
Clone
BLR047F
Host
Rabbit
Immunogen
Between 194 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Purified
Concentration
1000 µg/ml
Target Identity
TargetTIGIT
Antigen Species
Human
Gene ID201633
Gene Symbol
TIGIT
Gene Name
T-cell immunoreceptor with Ig and ITIM domains
Uniprot IDQ495A1
Protein Name
T-cell immunoreceptor with Ig and ITIM domains
Alternate Names
T-cell immunoreceptor with Ig and ITIM domains • V-set and immunoglobulin domain containing 9 • V-set and immunoglobulin domain-containing protein 9 • V-set and transmembrane domain containing 3 • V-set and transmembrane domain-containing protein 3 • VSIG9 • VSIG9, VSTM3 • VSTM3 • Washington University cell adhesion molecule • WUCAM
Product Information
Applications
Flow Cyt, IHC, IHC-IF, WB
Buffer
Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, BSA-Free
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Recombinant antibody was purified from cell culture supernatant.
Immunogen was a peptide representing a region between residue 194 and the C-terminus (residue 244) of human T-cell immunoreceptor with Ig and ITIM domains precursor (TIGIT) using the numbering given in entry NP_776160.2 (Gene ID 201633).
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Flow Cytometry (Flow Cyt)Fixed in 4% formaldehyde and permeabilized with 90% methanol. 2 µl per 1 x 10^6 cells.
Immunohistochemistry (IHC)1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Immunohistochemistry-Immunofluoresence (IHC-IF)1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Western Blot (WB)1:1000
Documents
Product DatasheetPDF · A700-047 · Lot 6 (current lot)Open
Product DatasheetPDF · A700-047 · Lot 6 (current lot)Open
Safety Data SheetPDF · A700-047Open
Citations / Publications
Services
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Antibody and conjugate formats developed together, built to your specification.
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Full custom monoclonal development, from immunization through validation.
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Single-domain antibodies for structurally difficult targets a conventional monoclonal can't reach.
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