Bethyl Laboratories
Rabbit anti-TIGIT Recombinant Monoclonal Antibody [BLR235K]
Monoclonal Antibodies
Catalog #A700-235
TargetTigit
HostRabbit
CloneBLR235K
ReactivityMouse
ApplicationWB
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Product Description
TIGIT is a member of the PVR (poliovirus receptor) family of immunoglobin proteins and is expressed on several classes of T cells including follicular B helper T cells (TFH). TIGIT has been shown to bind PVR with high affinity; this binding is thought to assist interactions between TFH and dendritic cells to regulate T cell dependent B cell responses. [taken from NCBI Entrez Gene (Gene ID: 100043314)].
Specifications
Clonality
Recombinant Monoclonal
Clone
BLR235K
Host
Rabbit
Immunogen
Residues 26-143 (ECD)
Isotype
IgG
Reactivity
Mouse
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Purified
Concentration
500 µg/ml
Target Identity
TargetTigit
Antigen Species
Mouse
Gene ID100043314
Gene Symbol
Tigit
Gene Name
T cell immunoreceptor with Ig and ITIM domains
Uniprot IDP86176
Protein Name
T-cell immunoreceptor with Ig and ITIM domains
Product Information
Applications
WB
Buffer
Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Recombinant antibody was purified from cell culture supernatant.
Immunogen was a recombinant protein representing the extracellular domain of mouse T-cell immunoreceptor with Ig and ITIM domains (TIGIT, residues 26-143, NP_001139797.1).
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western Blot (WB)1:1000
Documents
Product DatasheetPDF · A700-235 · Lot 1 (current lot)Open
Product DatasheetPDF · A700-235 · Lot 1 (current lot)Open
Product DatasheetPDF · A700-235 · Lot 220818Open
Safety Data SheetPDF · A700-235Open
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Resources
Standard Western Blot Protocol
Read the Protocol
Immunoprecipitation Protocol
Read the Protocol
Simple Western™ Resources
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