Bethyl Laboratories
Rabbit anti-TIM3 Recombinant Monoclonal Antibody [BLR207K]
Monoclonal Antibodies
Catalog #A700-207
TargetTIM3
HostRabbit
CloneBLR207K
ReactivityMouse
ApplicationIP, WB
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Product Description
TIM3 (HAVCR2) is a cell surface receptor implicated in modulating innate and adaptive immune responses. It is generally accepted to have an inhibiting function. Reports on stimulating functions suggest that the activity may be influenced by the cellular context and/or the respective ligand. TIM3 regulates macrophage activation, inhibits T-helper type 1 lymphocyte (Th1)-mediated auto- and alloimmune responses, and promotes immunological tolerance. In CD8+ cells TIM3 attenuates TCR-induced signaling, specifically by blocking NF-kappaB and NFAT promoter activities resulting in the loss of IL-2 secretion. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q8TDQ0].
Specifications
Clonality
Recombinant Monoclonal
Clone
BLR207K
Host
Rabbit
Immunogen
Between 231 and C-terminus
Isotype
IgG
Reactivity
Mouse
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Purified
Concentration
100 µg/ml
Target Identity
TargetTIM3
Antigen Species
Human
Gene ID171285
Gene Symbol
HAVCR2
Gene Name
hepatitis A virus cellular receptor 2
Uniprot IDQ8VIM0
Protein Name
Hepatitis A virus cellular receptor 2 homolog
Alternate Names
Product Information
Applications
IP, WB
Buffer
Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Recombinant antibody was purified from cell culture supernatant.
Immunogen was a peptide representing a region between residue 231 and the C-terminus (residue 281) of mouse Hepatitis A virus cellular receptor 2 (aka, TIM3) using the numbering given in entry NP_599011.2 (Gene ID 171285).
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)20 µl/mg lysate
Western Blot (WB)1:1000
Documents
Product DatasheetPDF · A700-207 · Lot 1 (current lot)Open
Product DatasheetPDF · A700-207 · Lot 1 (current lot)Open
Product DatasheetPDF · A700-207 · Lot 1 (current lot)Open
Safety Data SheetPDF · A700-207Open
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Resources
Standard Western Blot Protocol
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Immunoprecipitation Protocol
Read the Protocol
Simple Western™ Resources
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