Bethyl Laboratories
Rabbit anti-Timeless Antibody Affinity Purified
Primary
Catalog #A300-960A
TargetTimeless
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP
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Product Description
Timeless was first identified in Drosophila as a transcriptional regulator involved in the fly circadian rhythm. In mice, humans and yeast, the homologs of drosophila timeless have been observed to play a role in the DNA damage checkpoint response. Human timeless has been shown to interact with the circadian clock protein cryptochrome 2 as well as the DNA checkpoint proteins Chk1 and the ATR-ATRIP complex suggesting a link between circadian rhythm and the cell cycle.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1158 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetTimeless
Antigen Species
Human
Gene ID8914
Gene Symbol
TIMELESS
Gene Name
timeless circadian clock
Uniprot IDQ9UNS1
Protein Name
Protein timeless homolog
Alternate Names
Product Information
Applications
IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to Timeless immobilized on solid support.
The epitope recognized by A300-960A maps to a region between residue 1120 and 1170 of human Timeless using the numbering given in entry NP_003911.1 (GeneID 8914).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A300-960A · Lot 2 (current lot)Open
Product DatasheetPDF · A300-960A · Lot 2 (current lot)Open
Safety Data SheetPDF · A300-960AOpen
Citations / Publications
Services
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