Bethyl Laboratories
Rabbit anti-TIPIN Antibody Affinity Purified
Primary
Catalog #A301-474A
TargetTIPIN
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
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Product Description
Timeless-interacting protein (TIPIN) was originally isolated as a protein that interacts with timeless, a factor that plays a role in circadian rhythm and cell cycle checkpoint regulation. The TIM-TIPIN complex has been shown to coordinate the intra-S-phase checkpoint in response to UV-induced DNA damage. TIPIN is required for cell cycle arrest in response to DNA damage. It also interacts with RPA (replication protein A) on DNA and is able to slow down DNA chain elongation in response to UV-damage.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 251 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetTIPIN
Antigen Species
Human
Gene ID54962
Gene Symbol
TIPIN
Gene Name
TIMELESS interacting protein
Uniprot IDQ9BVW5
Protein Name
TIMELESS-interacting protein
Alternate Names
Product Information
Applications
IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to TIPIN immobilized on solid support.
The epitope recognized by A301-474A maps to a region between residue 251 and 301 of human TIMELESS interacting protein using the numbering given in entry NP_060328.1 (GeneID 54962).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A301-474A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-474A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-474AOpen
Citations / Publications
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